1) Custom Recombinant Protein Production and Purification

At BrinDx, we offer cutting-edge Custom Recombinant Protein.

We offer small to intermediate-scale production at very competitive prices, providing flexibility for various research needs.

• Production and Purification Services tailored to meet your research needs. Our comprehensive service encompasses every step of the process, ensuring high-quality results for your projects.
• Custom Protein Development: Our experienced team excels in intermediate-scale production of recombinant proteins. We provide custom protein expression, utilizing advanced techniques for optimal yields.
• Purification Expertise: Our one-stop service includes purification techniques that guarantee the isolation of pure and functional proteins. We employ state-of-the-art methods to ensure the highest quality of your desired protein.
• Quality Assurance: BrinDx prioritizes quality assurance throughout the workflow. From gene cloning to sequence validation, we maintain rigorous standards to deliver reliable and reproducible results for your research endeavors.
• Alexa-Tagged Proteins for Fluorescent Applications: Explore our Fluorescent-Labeled Proteins conjugated to Alexa Fluor dyes. These proteins are ideal for fluorescence microscopy, providing a better and brighter imaging experience.
• Endotoxin-Free Proteins: Addressing the challenge of this common contaminant in recombinant protein production, BrinDx also provides endotoxin-free options for specific research requirements.

2) Aggregation and seeding assays

BrinDx offers specialized services for alpha-synuclein (aSyn) and Tau aggregation and seeding assays. Our cutting-edge service provides a comprehensive screening platform to evaluate how compounds impact the amyloid aggregation of aSyn, Tau, and other neurodegenerative disease-relevant proteins.

• In vitro Assays: Utilizing recombinant proteins, our in vitro assays allow for precise examination of aSyn and Tau aggregation. This service enables the identification of compounds that modulate protein aggregation in controlled test environments, allowing the precise examination of aggregation kinetics, dose responses, seeding ability, the fibril disassembly in the presence of your candidate modulators.
• Cell-Culture Based Models: BrinDx extends its capabilities to cell-culture based models of neurodegenerative diseases, in partnership with INNOPROT. Our assays incorporate an array of relevant transgenic cell line to assess the impact of your compounds of interest on the amyloid aggregation, phosphorylation, and localization and of aSyn, Tau, TDP-43 among others, either in fixed samples by immunofluorescence coupled with high resolution confocal microscopy or directly within living cells, providing valuable insights into potential pharmaceutical lead compounds.

Choose BrinDx for precise and reliable aggregation and seeding assays to advance your research in neurodegenerative diseases.

3) aSyn oligomer toxicity assays 

Recent research has unveiled a critical insight into Parkinson’s disease (PD), highlighting the pivotal role of dopamine-stabilized alpha-synuclein (aSyn) oligomers as a primary toxic species.This underscores the importance of evaluating the effect of potential drug candidates on dopamine-stabilized aSyn oligomers, in order to shedd light on novel therapeutic avenues for targeting the specific molecular mechanisms implicated in PD progression.

BrinDx offers a distinctive capability to assess the impact of compounds on alpha-synuclein (aSyn) oligomer toxicity. Our aSyn dopamine-stabilized oligomers, designed for accurate toxicity evaluations, possess unique characteristics:

• ThT Negative: The oligomers exhibit a Thioflavin T (ThT) negative profile, indicating a non-amyloidogenic nature and differentiating them from traditional amyloid structures.
• Size: Measured by Dynamic Light Scattering (DLS), our aSyn dopamine-stabilized oligomers have a consistent size of 100 nm, ensuring reliability and uniformity in toxicity assessments.
• Toxic: These oligomers demonstrate pronounced toxicity specifically to SH-SY5Y cells, providing a relevant cellular model for neurodegenerative studies.
• SDS Resistant: Our oligomers exhibit resistance to Sodium Dodecyl Sulfate (SDS), ensuring stability and reproducibility in experimental settings.

Choose BrinDx for precise and reliable evaluations of compound effects on aSyn oligomer toxicity, utilizing our unique dopamine-stabilized oligomers.

4) General cytotoxicity assays

BrinDx provides basic cytotoxicity assays, including MTT and LDH, in a wide variety of wilt-type and transgenic cell lines. These assays are valuable tools for assessing cellular viability and detecting cytotoxic events. The MTT assay measures cell viability by assessing the reduction of MTT to formazan, while the LDH assay quantifies cytotoxicity by measuring lactate dehydrogenase released from damaged cell membranes. Both assays play crucial roles in understanding cellular responses to various compounds and evaluating potential toxic effects.

5) Biophysical and biochemical characterization of neurodegenerative disease-relevant protein aggregates

BrinDx offers comprehensive services for the biophysical and biochemical characterization of neurodegenerative disease-relevant protein aggregates. Thorough evaluation includes techniques such as:

• TEM (Transmission Electron Microscopy): High-resolution imaging to visualize protein aggregates at the nanoscale.
• SEM (Scanning Electron Microscopy): Provides detailed three-dimensional surface images of protein aggregates.
• DLS (Dynamic Light Scattering): Measures the size distribution of protein aggregates in solution.
• Electrophoresis: Evaluates the migration pattern of protein aggregates based on charge and size.
• Congo Red Staining: Utilizes Congo Red dye for histological detection of amyloid aggregates.
• ThT (Thioflavin-T) Fluorescence Assay: Quantifies amyloid content in protein aggregates.
• Cellular uptake: Assesses the cellular internalization of protein aggregates.
• Seeding Ability in Cellula and In Vitro: Examines the ability of protein aggregates to induce aggregation in cells and in vitro.
• Bis-ANS Staining: Measures the exposure of hydrophobic regions in protein aggregates.